牛磺酸的非内皮依赖性舒血管作用机制

Endothelium-independent vasorelaxant effect of Taurine on rat aorta rings

目的:研究牛磺酸(Tau)对血管的舒张作用并探讨其机制。方法:采用大鼠离体主动脉环灌流模型,经生物信号采集与分析系统测定Tau 10,20,40,80 mmol.L-1对苯肾上腺素(PE)1μmol.L-1和氯化钾(KCl)60 mmol.L-1预收缩内皮完整和去内皮血管的舒张作用,及Tau对不同工具药血管反应性的影响。结果:Tau能非血管内皮依赖性舒张由PE和KCl诱导收缩的大鼠胸主动脉环;对PE诱发的依赖内钙释放和外钙内流的血管环收缩均有抑制作用;对咖啡因诱导的血管环收缩无明显抑制作用;格列苯脲和四乙胺能够明显抑制Tau的舒张血管作用,但氯化钡和4-氨基吡啶不能抑制其舒张血管作用。结论:Tau的血管舒张作用是非内皮依赖性的,其机制可能是通过抑制血管平滑肌依内Ca2+性收缩(IP3介导)和依外Ca2+性收缩起作用的。同时KATP通道和KCa通道可能参与了Tau的舒血管作用。

Objective:To investigate the vasorelaxant effect of taurine(Tau) in rat aortic rings and the mechanism.Method:The isolated thoracic aortic rings of male Wistar rats were mounted on the organ bath.The effect of Tau 10,20,40,80 mmol.L–1 on the rings with endothelium intact or endothelium denuded precontracted by the phenylephrine(1 μmol.L-1) or KCl(60 mmol.L-1),and the effect of Tau on the vessel reaction induced by various drugs were recorded with biological signal analytical system.Result:Taurine completely...