超临界流体萃取党参中党参炔苷的研究

Extraction of lobetyolin from codonopsis with supercritical CO_2

目的:党参炔苷是党参中开发一条绿色快速的工艺路线,提取党参中的重要的有效成分之一党参炔苷(lobetyo-lin)。方法:本实验对党参中党参炔苷的超临界CO2萃取工艺进行了研究。采用中心实验设计结合响应曲面法考察了压力、温度、萃取时间和夹带剂用量等因素对党参炔苷得率的影响及其交互作用。结果:确定了夹带剂用量是关键因素,在动态夹带剂乙醇流速为1 mL.min-1,常压CO2流速为2 L.min-1,30 MPa,60℃下条件下萃取100 min,党参炔苷得率为0.078 6 mg.g-1,优于传统超声萃取法。结论:采用带夹带剂的超临界流体萃取党参中党参炔苷是可行的。

Objective: To develop a green and rapid method for extraction of lobetyolin from C.pilosula.Method: Extraction of lobetyolin from C.pilosula with supercritical carbon dioxide in the presence of ethanol was studied.The effects of pressure,temperature,volume of cosolvent and extraction time on efficiency and their interactive relationships were discussed,based on central composite design and response surface methodology(RSM).Result: The key effect factor was volume of cosolvent.The extraction yield of lobetyo...