三七总皂苷对大鼠主动脉球囊损伤后血管内膜增生及PCNA表达的影响

Effect of Panax notoginseng saponins on vascular intima hyperplasia and PCNA expression in rat aorta after balloon angioplasty

目的:研究三七总皂苷(PNS)对大鼠主动脉球囊损伤后血管内膜增生及增殖细胞核抗原(PCNA)表达的影响,阐明其作用机制。方法:以2.0 F球囊导管建立大鼠主动脉内膜剥脱模型,随机分为假手术组、模型组、三七总皂苷(100 mg.kg-1组、阳性药阿托伐他汀(10 mg.kg-1)组。造模后第2天开始给药,连续给药14 d后取胸主动脉损伤段,以组织形态学和免疫组织化学方法测定血管形态计量指标及PCNA表达。结果:模型组的内膜面积、内膜厚度、内膜面积增生比率、内膜厚度增生比率、内膜/中膜面积比、内膜/中膜厚度比均显著大于假手术组(P<0.01)。PNS组和阿托伐他汀组上述各指标均低于模型组(P<0.05)。模型组增生内膜中PCNA表达较假手术组显著增强(P<0.01)。PNS组、阿托伐他汀组PCNA表达均显著低于模型组(P<0.01)。结论:PNS可抑制血管内膜损伤后引起的血管内膜增生,其作用可能是通过抑制血管内膜损伤后血管平滑肌细胞增殖而实现的。

Objective:To explore the effect and the mechanism of panax notoginseng saponins(PNS) on the vascular intima hyperplasia and expression of proliferating cell nuclear antigen(PCNA) after aortic intima injury induced ballcon in rats.Method:Model of aortic intima denudation in rats was established by 2.0 forgarty.The rats were randomly divided into sham group,model group,PNS group and atorvastatin group.Drugs were administered at the second day after the aortic intima injury for 14 days.The injured thoracic aor...