用于基因传输及磁共振显像的聚乙烯亚胺-g-聚己内酯共聚物的合成与表征

SYNTHESIS AND CHARACTERIZATION OF POLYETHYLENIMINE-g-POLY(ε-CAPROLACTONE) COPOLYMER FOR GENE DELIVERY AND MAGNETIC RESONANCE IMAGING

采用可生物降解的聚己内酯改性聚乙烯亚胺,得到两亲性的接枝共聚物(PEI-g-PCL).该共聚物通过溶剂挥发法在水中自组装形成纳米粒子,其内部负载有超顺磁性四氧化三铁纳米粒子(SPIO)及质粒DNA(pDNA).研究表明,PEI-g-PCL聚合物自组装形成的颗粒为胶束状,无论是否负载SPIO纳米粒子都可以有效地负载pDNA,并对293细胞具有较高的转染效率.此类载体有望在基因转染的过程中利用磁共振手段进行实时、无创观测.

Gene therapy is a promising approach to treating diseases that caused by genetic defects or mutation.Non-invasive imaging of gene is a powerful tool in understanding the biodistribution and pharmacokinetics of vectors after in vivo administration.In the current research,amphiphilic copolymers,which were utilized to deliver a magnetic resonance imaging(MRI) contrast agent and pDNA simultaneously,were obtained by modifying hyperbranched polyethylenimine with biodegradable hydrophobic segment(PCL).Synthesis of...