HPLC法测定复方酮康唑乳膏的含量

Determination the Contents of Ketoconazole and Clobetasol Propionate in Compound Ketoconazole Cream by RP-HPLC

目的:建立RP-HPLC法测定复方酮康唑乳膏(皮康王)中的酮康唑、丙酸氯倍他索的含量。方法:用THERO Hylicerm ODS C_(18)(250mm×4.6mm,5μm),流动相为甲醇-0.02mol·L^(-1)磷酸二氢钾溶液(72:28)(用氨水调pH到7.2),流速1. 0ml·min^(-1),检测波长240nm。结果:酮康唑和丙酸氯倍他索的线性范围分别为2.5～25.0μg(r=0.999 9)和0.07～0.66μg(r =0.999 8),平均回收率分别为100.0%(RSD 0.1%)和99.6%(RSD 0.4%)(n=6)。结论:方法专属和准确,可作为复方酮康唑乳膏的质量控制。

Objective:To establish a RP-HPLC method for determination the contents of ketoconazole and clobestasol propionate in compound ketoconazole cream.Method:The thero hylicerm ODS-C_(18) column(250mm×4.6mm,5μm) was used,the mobile phase consisted of methanol -0.02mol·L^(-1) potassium dihydrogen phosphate solution(72:28),the flow rate was 1.0 ml·min^(-1),the detection wavelength was 240 nm.Result:The linear ranges of ketoconazole and clobetasol propionate were 2.5-25μg(r=0.999 9) and 0.07 -0.66μg(r=0.999 8),the a...