高效液相色谱法测定凉粉草中熊果酸的含量

Determination of ursolic acid in the mesona chinensis benth by high performance liquid chromatography

试验建立了高效液相色谱法测定凉粉草熊果酸含量的方法。采用C18柱,流动相为甲醇,流速为1 mL·min^(-1),检测波长为210 nm。进样浓度在0.003 2～0.032 mg·mL^(-1)范围内线性关系良好,平均回收率为91. 9%。试验结果显示,凉粉草中含有熊果酸,并测得不同样品的熊果酸含量3.911～3.915 mg·g^(-1)之间。

A HPLC method was established for the determination of Ursolic Acid in Mesona chinensis Bentht.C18 column is used,the flowing is the methyl alcohol,the examination wave length is 210 nm.The specimen handling density is good in the 0.003 2～0.032 mg/mL scope,the average returnratio is 91.9%.The test result shows that ursolic acid is included in the immortal grass,the different sample ursolic acid content is between 3.911～3.915 mg/g.