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脐血源性T淋巴细胞移植后Caspase-3和Bcl-2在红白血病小鼠脾内的表达

The Expressions of Caspase-3 and Bcl-2 Protein in Spleen of Erythroleukemic Mice after Cord Blood-Derived T Lymphocytes Transplantation
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摘要 目的研究脐血源性T淋巴细胞移植入红白血病小鼠体内后脾脏Caspase-3和Bcl-2蛋白的表达状况,探讨脐血源性T淋巴细胞对白血病细胞的杀伤作用。方法V给EL9611红白血病小鼠尾静脉注射或腹腔注射脐血源性T淋巴细胞,于第7天时处死,取脾脏,用免疫组化法检测Caspase-3及Bcl-2蛋白表达。结果实验组与对照组比较,Caspase-3阳性表达增强(P<0.05),Bcl-2阳性表达降低(P<0.05)。结论脐血源性T淋巴细胞能诱导白血病细胞凋亡,对白血病有一定的治疗作用;其机制可能与Caspase-3活化、Bcl-2功能被抑制有关。 Objective To study the effect of the expression of Caspase-3 and Bcl-2 protein in spleen of erythroleukemic mice after cord blood-derived T lymphocytes transplantation,and to investigate its role of anti-leukemia.Mdthods E19611 erythroleukemia mice were injected with cord blood-derived T cells from vena caudalis.After seven days,the mices were sacrificed and their spleen were taken out.Then the expressions of Caspase-3 and Bcl-2 protein were observed by immunohistochemical staining method analysis.Results C...
作者 耿微 张圣明 张福琴 石娟 潘淑杰
机构地区 潍坊医学院组织胚胎学教研室 潍坊医学院附属潍坊市人民医院产科
出处 《潍坊医学院学报》 2008年第6期-,共4页 Acta Academiae Medicinae Weifang
关键词 脐血源性T淋巴细胞 CASPASE-3 BCL-2 凋亡 免疫组织化学 Cord blood-derived T lymphocyte Caspase-3 Bcl-2 Apoptosis Immunohistochemistry
分类号 R733.7 [医药卫生—肿瘤]
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潍坊医学院学报
2008年 第6期

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