支气管扩张症患者痰中IL-8和TNF-α测定的意义

Significance of Detecting IL-8 and TNF-αin Sputum from Patients with Bronchiectasis

目的探讨支气管扩张症患者急性发作期以及病情缓解后痰液中中性粒细胞占白细胞百分比(Neu/Leu%)、白细胞介素8(IL-8)、肿瘤坏死因子α(TNF-α)的变化及与肺功能的相关性。方法对45例支气管扩张症急性发作期患者治疗前及10～14d病情缓解后分别行肺功能检查测定FEV1.0占预计值百分数(FEV1.0pre)、用酶联免疫吸附法(ELISA)法检测诱导痰液中IL-8和TNF-α的水平,并记录诱导痰液Neu/ Leu%.结果支气管扩张症急性发作期痰液中Neu/Leu%,IL-8和TNF-α水平明显高于缓解后患者和健康对照者,差异有统计学意义(P<0.05),支气管扩张症患者缓解期痰液中Neu/Leu%,IL-8和TNF-α水平均明显高于健康对照者,差异有统计学意义(P<0.05),支气管扩张症患者急性发作期以及病情缓解后痰液Neu/ Leu%,IL-8与FEV1.0pre呈负相关。结论痰液Neu/Leu%,IL-8,TNF-α参与了支气管扩张症气道炎症过程,而且痰液Neu/Leu%,IL-8和气道阻塞相关。

Objective To study the variation of the proportion of neutrophils/leukocytes(Neu/Leu%),the level of serum interleukin (IL)-8,and tumour necrosis factorα(TNF-α)in sputum of patients with broniectasis both during acute episodes and after disappearance of symptoms,and the relationship between the pulmonary function and the airway inflammation of broniectasis.Methods The induced sputums from 45 patients with bronchiectasis in acute exacerbations and the stable period with treatment for 10～14 days were collected...