黄芪对肝纤维化大鼠NF-κB的调节作用

Effect of Astragalus on NF-κB in Liver Fribrosis Rat

目的通过观察NF-κB表达情况,探讨黄芪抗肝纤维化机制。方法45只SD大鼠随机分为正常对照组、CCl_4诱导肝纤维化模型组和黄芪干预组。HE染色评价各组肝纤维化程度,免疫组化方法检测NF -κB在肝组织中表达,利用单因素方差分析法研究其表达水平与肝纤维化关系。结果HE染色结果显示,肝纤维化模型组的肝纤维化分级明显高于正常对照组(P<0.01),黄芪组肝纤维化分级与肝纤维化模型组比较明显降低(P<0.01);肝纤维化模型组大鼠肝组织NF-κB表达显著高于正常对照组(P<0.01),而黄芪组NF-κB表达较模型组显著降低(P<0.01)。结论黄芪可以显著抑制CCl_4诱导的肝纤维化,对NF-κB信号转导通路相关分子的调控可能是其机制之一。

Objective To study on the effect of astragalus on NF-κB signal in rat model of liver fribrosis. Methods Forty-five SD rats were randomly divided into normal control group,carbon tetrachloride(CCl_4)-induced liver fibrosis model group and Astragalus group.CCl_4 was injected subcutaneously for ten weeks,and all rats were killed immediately after CCl_4 intervention.HE staining was used to measure the degree of liver fibrosis.Immunohistochemistry was used to measure the expression of NF-κB in liver tissue.One-w...