高加索三叶草和白三叶草再生体系的建立

THE ESTABLISHMENT OF EFFICIENT REGENERATION SYSTEM FOR CAUCASIAN CLOVER AND WHITE CLOVER

高加索三叶草和白三叶草在饲料牧草中占有重要地位。本实验以其茎和叶为外植体材料,比较不同外植体的再生能力,探讨不同激素组合对愈伤组织诱导、继代培养以及植株分化的影响。结果表明茎的再生能力比叶片强。高加索三叶草在MS+2,4-D0.25?/L+6BA0.5/L的培养基中愈伤组织诱导生长最好,愈伤组织在MS+2,4-D0.2/L+6BA1.0/L+2%蔗糖培养基上生长迅速,质地良好,在MS+2,4-D 0.25/L+6BA 1.0/L培养基上植株分化率达75%;白三叶草在MB+在MB+2,4-D2.0/L+6BA 0.75/L培养基上诱导出完整植株。

Caucasian clover and white clover play an important role in animal feed herbage.Stems and leaves were used as explants to test the regeneration ability and the study the effects of different hormone compositions,proliferation and plantlet regeneration.The results showed that the regeneration ability of stems was higher than that of leaves.MS+2,4-D0.25㎎/L +6BA0.5㎎/L+3%sucrose,MS+2,4-D0.2㎎/L+6BA1.0㎎/L+2% sucrose,MS+2,4-D 0.25㎎/L+6BA 1.0㎎/L were better for callus induction,proliferation and regeneration of Cau...