绵羊肺腺瘤病实验室诊断报告

LABORATORY ON THE DIAGNOSES OF OVINE PULMINARY ADENOMATOSIS

以乌兰察布市某种羊场的疑似病羊为研究对象,采用临床诊断、镜检观察及PCR法检测患病羊是否患有绵羊肺腺瘤病。结果显示:①"小推车试验"时有鼻液流出;②剖检时可见肺实变,体积明显增大,表面出现大量形态不规则的灰白色结节,切开肺脏时可见大量泡沫状液体从切面渗出;③显微镜下观察,疑似患病羊的肺脏有大小不一的腺瘤灶,且肺泡上皮细胞呈乳头状增生,肺泡腔内充满巨噬细胞,病灶中央的细胞核溶解,胞浆中有空泡,呈坏死状;④根据GenBank中已发表的外源性绵羊肺腺瘤病毒的基因组全序列,设计合成3对特异性引物,对病料样品进行PCR扩增并将其产物测序,结果表明扩增出的片段为178bp、226bp、301bp,同源性分析表明这3段序列与引起该病的病原即绵羊肺腺瘤病毒基因组序列同源性最高分别为97.2%、96.5%、94.4%,与相对应的内源性病毒序列最高分别为73%、69.5%、85.4%,以上4方面诊断该病例为绵羊肺腺瘤病。其中所建立的PCR诊断方法具有快速、特异、敏感等特点,为绵羊肺腺瘤病的快速诊断提供了新的手段。

In order to diagnosis the ovine who was fell adenomatosis,the Wulanchabu city ovine were studied by clinical diagnosis,Microscopic exam observation and PCR.The result showed that: ①the blenna narium flueffused when"wheel-barrow test";②the lunge was larger than normal.The surface showed many gray nodus,a lot of cystose fluid was effusion from cut surface;③in microscope observed,many adenoid focus were in the lung,the alveolar epithelial cell accrementied like corpora mammillaria.The macrophage were fulled in...