期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

前列腺癌演进过程中的等位基因失衡 被引量：1

Allelic Imbalance in the Clonal Evolution of Prostate Carcinoma
下载PDF
导出
摘要 目的了解前列腺癌克隆演变(clonal evolution)过程中的遗传学机制。方法采用激光显微切割技术从保存的石蜡包埋组织中获取基因组DNA;利用6个位于染色体8p12-21、8p22、17q21上的具有多态性的微卫星标记,对25例患者原发癌及相应转移灶中等位基因的缺失或保留进行分析。结果在24例信息病例中,14例(58%)在原发癌及相应转移灶中所有位点均表现为相同的等位基因缺失或保留模式,而另外10例(42%)则显示不一致的等位基因缺失。10例中有8例原发癌表现为等位基因保留,而在相应转移灶则为缺失,另外2例在一个或一个以上的位点表现为不同的等位基因缺失模式。结论前列腺癌在原发癌及相应转移灶遗传组成上的差异可能与其内在异质性、整体遗传不稳定性及克隆差异有关。 Objective To observe the genetic basis of the clonal evolution of prostate carcinoma.Methods The pattern of allelic loss in 25 matching primary and metastatic prostatic adenocarcinoma were analyzed.DNA sample for the analysis of allelic loss pattern were prepared by tissue microdissection.The oligonucleotide primer pairs for the microsatellite DNA markers were D8S133,D8S136,D8S137,ANK1 on chromosome 8p12-21,LPLTET on chromosome 8p22,and D17S855 on chromosome 17q21.Results The overall frequency of allelic im...
作者 李光 张海峰 徐妙生
机构地区 首都医科大学附属北京天坛医院病理科 山西医科大学第一临床医学院病理科
出处 《首都医科大学学报》 CAS 北大核心 2009年第2期239-242,共4页 Journal of Capital Medical University
关键词 前列腺 转移 等位基因失衡 显微切割 肿瘤进展 prostate metastasis allelic disequilibrium microdissection tumor progression
分类号 R737.25 [医药卫生—肿瘤]
  • 相关文献

参考文献10

  • 1Woodhouse EC,Chuaqui RF,Liotta LA.General mechanisms of metastasis[].Cancer.1997
  • 2Vocke C D,Pozzatti R O,Bostwick D G,et al.Analysis of 99 microdissected prostate carcinomas reveals a high frequency of allelic loss on chromosome 8p12-21[].Cancer Research.1996
  • 3Suzuki H,Freije D,Nusskern DR,et al.Interfocal heterogeneity of PTEN/MMAC1 gene alterations in multiple metastatic prostate cancer tissues[].Cancer Research.1998
  • 4Frost,P,Levin,B.Clinical implication of metastatic process[].The Lancet.1992
  • 5Zhuang ZP,Bertheau P,Emmert-Buck MRetal.Aml- crodissection technique for archival DNA analysis of specific cell popuation in lesions≤1 mm in size[].American Journal of Pathology.1995
  • 6Dean-Clower E,Vortmeyer AO,Bonner R F,et al.Micro-dissection-based genetic discovery and analysis applied to cancer progression[].Cancer Jsci Am.1997
  • 7Qian J,Bostwick DG,Takahashi S,et al.Chromosomal anomalies inprostatic intraepithelial neoplasia and carcinoma detected by fluorescencein situ hybridization[].Cancer Research.1995
  • 8Bostwick D G,Shan A,Qian J,et al.Independent origin of multiple foci of prostatic intraepithelial neoplasia(PIN):comparison with matched foci of prostate cancer[].Cancer.1998
  • 9Bonsing BA,Devilee P,Cleton-Jansen A,Kuipers-Dijkshoorn N,Fleuren GL,Cornelisse CJ.Evidence for limited molecular genetic heterogeneity as determined by allelotyping and clonal analysis in nine metastatic breast carcinomas[].Cancer Research.1993
  • 10Chen,LC,Kurisu,W,Ljung,BM,Goldman,DS,Moore,D,Smith,HS.Heterogeneity for allelic loss in human breast cancer[].Journal of the National Cancer Institute.1992

同被引文献9

  • 1Pelicano H, Carney D, Huang P. ROS stress in cancer cells and thera-peutic implications [J]. Drug Ressist Updat,2004,7(2):97-110.
  • 2Frohlich DA,Mccabe MT,Arnold RS,et al. The role of Nrf2 in in- ereased reactive oxygen species and DNA damage in prostate tumori- genesis[ J ]. Oncogene, 2008,27 ( 31 ) : 4353-4362.
  • 3Ishii T,Itoh K,Takahashi S,et al. Transcription factor Nrf2 coordi- nately regulates a group of oxidative stress-inducible genes in macrophages [J]. J Biol Chem, 2000,275 (21 ) : 16023-16029.
  • 4Tsutsui H, Kinugawa S, Matsushima S. Mitoehondrial oxidative stress and dysfunction in myocardial remodelling [J]. Cardiovasc Res, 2009,81(3) :449-456.
  • 5Stacy DR,Ely K,Massion PP,et al. Increase expression of nuclear Factor E2 p45-related factor 2 (NRF2) in head and neck squamous cell carcinomas[ J ]. Head Neck, 2006,28 ( 9 ) : 813-818.
  • 6Kin JH, Bogner PN. Elevated penoxiredoxin 1, but not NF-E2-related factor 2, is an independent prognostic factor for disease recurrence and reduced survival in stage I non-small cell lung cancer [J]. Clin Cancer Res, 2007, 13 ( 13 ) : 3875-3882.
  • 7] Cho JM, Manandhar S,Lee HR,et al. Role of the Nrf2-antioxidant system in cytotoxicity mediated by anticancer cisplatin:implication to cancer cell resistance[ J ]. Cancer Lett, 2008,260( 1-2 ) : 96-108.
  • 8杨海明,张明鑫,王建生,等.原发性胆囊癌巾Nvf2的表达及意义[J].现代肿瘤学杂志,2010,18(4):745-747.
  • 9Bin H, Hiroto I, Yoshihiro Y, et al. Human mitochondrial transcrip- tion factor A functitms in both hue|el and mitochondria and regulate cancer cell growth [J]. Biochem Biaphys Res Commun,2011,408 (l):45-51.

二级引证文献3

首都医科大学学报
2009年 第2期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部