高效液相色谱法测定强力枇杷露中吗啡的含量

Determination of Morphine in Qiangli Pipa Syrup by HPLC

目的建立强力枇杷露中吗啡的含量测定方法。方法采用HPLC法,C8柱(150mm×4.6mm,5μm),以乙腈-0.01moL?L-1磷酸氢二钾溶液-0.005moL?L-1庚烷磺酸钠溶液(19:41:40)为流动相,检测波长为220nm;柱温25℃,流速1.0mL?min-1。结果吗啡在5.015～80.240μg?mL-1范围内线性关系良好(r=0.9999),平均回收率97.95%,RSD=1.0%(n=6)。结论本方法操作简便、重现性好;可准确测定强力枇杷露中吗啡的含量。

Objective To establish a method for the determination of morphine in Qiangli Pipa syrup.Methods XDB-C8 (150 mm×4.6 mm,5 μm) was used with the column temperature of 25 ℃ , acetonitrile-0.01 mol-L-1 K2HPO4-0.005 mol-L-1 C7H15NaO3(19:41:40)as mobile phase, the detective wavelength was at 220nm, the flow rate was 1.0 mL-min-1. Results Morphine was linear in the range of 5.015～80.240 μg-mL-1(r=0.999 9), the average recovery was 97.95 % with RSD of 1.0%(n=6). Conclusion The method is simple,accurate and reproduci...