超声波法优化酒糟中玉米黄色素的提取工艺及其抗肿瘤的研究

Optimization of the Ultrasonic Assisted Extraction Conditions of Maize Yellow Pigment in Distiller's Grains Using Response Surface Methology and the Studying on anti-neoplasms Effect

采用响应面法优化超声波提取酒糟中玉米黄色素的提取条件并对超声提取玉米黄色素进行抗肿瘤活性研究。在单因素试验的基础上,选取超声时间、固液比和超声波功率为关键因子,应用Box-Behnken中心组合进行3因素3水平的实验设计,以色素收率作为响应值,进行响应面分析(RSA)。结果表明,超声波法提取酒糟中玉米黄色素的最佳提取条件为:提取时间69.85min,超声功率744W,固液比1∶4.5(g:mL),提取率预测值137μg/g,验证值为132μg/g,与预测值的相对误差为3.8%;超声提取的酒糟玉米黄色素对Hela细胞、乳腺癌细胞具有较好的抑制作用。

The response surface analysis methodology(RSM) was used for optimizing ultrasonic assisted extraction conditions in the extraction process of maize yellow pigment.And the anti-neoplasms is studied on the maize yellow pigment extracted by ultrasonic.Base on the single-factor experiments,three independent variables,Extracting time,water-solid ratio and ultrasonic power,were selected as casual factors during extraction.The experiments were arranged according to Box-Behnken central composite experiment design.R...