用于输送siRNA的聚乙烯亚胺-聚乙二醇二丙烯酸酯纳米凝胶

SYTHESIS AND CHARACTERIZATION OF POLYETHYLENIMINE-POLY(ETHYLENE GLYCOL) DIACRYLATE NANOGEL AS A siRNA CARRIER

阐述了一种新的用于输送小干扰核糖核酸(siRNA)的聚乙烯亚胺-聚乙二醇二丙烯酸酯纳米凝胶的合成及其表征.在反相微乳液体系中,聚乙烯亚胺(PEI)和聚乙二醇二丙烯酸酯(PEG-DA)通过麦克尔加成交联得到纳米凝胶.1H-NMR和FTIR的结果充分证明纳米凝胶的化学结构,而动态光散射则表明纳米凝胶的颗粒粒径均匀,约为180 nm,zeta电位为37.9 mV.同时,MTT结果表明纳米凝胶在高达1 mg/mL的浓度下对MCF-7细胞也基本没有毒性.凝胶阻滞实验证明纳米凝胶在体外可以通过静电相互作用稳定结合siRNA.转染实验结果表明纳米凝胶与GFP siRNA的复合物能降低MCF-7 KMRV细胞(一种能稳定表达GFP蛋白的MCF-7细胞系)GFP蛋白的表达.以上结果证明这种生物相容性的纳米凝胶可以结合并输送siRNA进入细胞,沉默相关基因的表达.

Delivery of synthetic small interfering RNA(siRNA) remains the major obstacle to the therapeutic application of RNA interference.A novel nanogel that composed of hydrophilic polyethylene glycol and cationic polyethylenimine for siRNA delivery was synthesized and characterized.The nanogel was synthesized via inverse microemulsion polymerization of polyethylenimine with M_w=800 and poly(ethylene glycol) diacrylate(M_w=400).The successful synthesis of the nanogel was demonstrated by^1H-NMR,FTIR characterizatio...