摘要
以地瓜生粉作为培养基的主要成分,为酵母菌的生长提供碳源,运用紫外线照射培养出的酵母菌,使其发生突变,经过初筛、复筛、驯化,培养出一株高产酒精的突变酵母菌S604(紫外线照射时间为60s,筛选4轮),并通过正交试验筛选出最优化培养基,在最优化培养基上培养突变菌株,测得其产酒精量比FCC2129高。
In this study, sweet potato meal was chosen as the principal constitute of culture medium to provide carbon source for the growth of yeast (Saccharomyces cerevisiaes). The mutation of original yeast was induced by UV radiation. After primary screening, secondary screening and domestication, a mutant of high alcohol-producing yeast S604 was obtained via 60-s UV radiation and 4-round screening. Then the culture medium composition of the mutant strain was optimized by orthogonal test. The fermntation experimen...
出处
《食品科学》
EI
CAS
CSCD
北大核心
2009年第5期187-189,共3页
Food Science
基金
国家自然科学基金项目(30570445)
辽宁省教育厅A类攻关项目(2004D238)
关键词
酵母菌
诱变
酒精
正交试验
yeast
sweet potato meal
mutation
alcohol
orthogonal test
