吸烟对2型糖尿病患者血清细胞黏附分子水平和氧化应激的影响

Relationship between Cell Adhesion Molecules Levels and Oxidative Stress in Male Smokers with Type 2 Diabetes

目的探讨吸烟对男性2型糖尿病(T2DM)患者血清细胞黏附分子(CAM)水平和氧化应激的影响。方法选择112例男性T2DM患者,其中吸烟T2DM患者63例,非吸烟T2DM患者49例,另选择30例男性非吸烟健康者为对照组。检测3组受检者血清可溶性细胞间黏附分子-1(sICAM-1)、可溶性血管细胞黏附分子-1(sVCAM-1)、E-选择素、丙二醛水平及超氧化物歧化酶(SOD)活性。结果吸烟T2DM组患者血清丙二醛、sICAM-1、sVCAM-1水平及SOD活性〔分别为(8.2±3.6)、(1033±201)μg/L、(2.2±1.5)μg/L、(71±10)U/ml〕与对照组〔分别为(4.2±2.4)μmol/L、(579±112)μg/L、(1.4±0.9)μg/L和(105±16)U/ml〕及非吸烟T2DM组〔分别为(6.5±4.9)μmol/L、(899±121)μg/L、(1.4±0.8)μg/L和(82±11)U/ml〕比较,差异均有统计学意义(P<0.05)。吸烟T2DM患者和非吸烟T2DM患者E-选择素水平〔(62±28)μg/L和(59±19)μg/L〕与对照组〔(41±15)μg/L〕比较,差异均有统计学意义(P<0.05)。重度吸烟T2DM组患者血清sICAM-1、丙二醛水平〔分别为(1119±188)μg/L、(9.5±3.4)μmol/L〕及SOD活性〔(61±10)U/ml〕与非吸烟T2DM组〔分别为(899±121)μg/L、(6.5±4.9)μmol/L和(82±11)U/ml〕、轻度吸烟T2DM组〔分别为(938±137)μg/L、(7.6±2.4)μmol/L和(77±9)U/ml〕、中度吸烟T2DM组〔分别为(986±151)μg/L、(8.3±2.5)μmol/L和(67±12)U/ml〕比较,差异均有统计学意义(P<0.05);中度吸烟T2DM组患者血清sICAM-1、丙二醛水平及SOD活性与非吸烟T2DM组、轻度吸烟T2DM组比较,差异亦均有统计学意义(P<0.05)。吸烟T2DM患者血清sICAM-1水平与收缩压、胰岛素抵抗指数(HOMA-IR)及丙二醛、E-选择素水平呈正相关(r值分别为0.36、0.29、0.38、0.28,P<0.05),与SOD活性呈负相关(r=-0.25,P<0.05);血清sVCAM-1水平与收缩压、HOMA-IR、丙二醛水平呈正相关(r值分别为0.25、0.28、0.32,P<0.05);E-选择素水平与HOMA-IR、sICAM-1、丙二醛水平呈正相关(r值分别为0.31、0.28、0.30,P<0.05),与SOD活性呈负相关(r=-0.22,P<0.05)。结论吸烟可增加男性T2DM患者血清CAM水平及氧化应激,且吸烟量与sICAM-1和氧化应激水平相关。

Objective To investigate the relationship between cell adhesion molecules(CAM) levels and oxidative stress(OS) in male smokers with type 2 diabetes mellitus(T2DM).Methods This study included 112 male T2DM patients,63 of whom were smokers,49 nonsmokers;another 30 healthy male nonsmokers were chosen as control group.We detected the levels of soluble intercellular adhesion molecule-1(sICAM-1),soluble vascular cell adhesion molecule-1(sVCAM-1),E-selectin and malondialdehyde(MDA),and activity of superoxide dismu...