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巴西橡胶树6个小G蛋白基因cDNA的克隆与序列分析 被引量：2

Molecular Cloning and Sequence Analysis of 6 cDNAs Encoding Small GTPases from the Latex of Hevea brasiliensis
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摘要 利用笔者实验室建立的橡胶树胶乳EST序列数据库,结合RT-PCR和RACE技术,克隆巴西橡胶树6个小G蛋白基因的cDNA,并以拟南芥小G蛋白家族基因为模板进行进化树分析。结果表明:所克隆的基因分属于小G蛋白的Arf与Rab家族,将其分别命名为HbArf1、HbArf2、HbRab1、HbRab2、HbRab3和HbRab4。氨基酸序列分析发现,HbRab1、HbRab2、HbRab3和HbRab4均具有小G蛋白家族的保守结构域,即4个鸟苷酸(GTP/GDP)结合域、1个效应区和C端半胱氨酸结构域,而HbArf1和HbArf2除在C端无半胱氨酸结构域及效应区序列保守性差外,HbArf1还缺少2个鸟苷酸结合区,HbArf2缺少1个鸟苷酸结合区。 Small GTPases in plants belong to a gene superfamily, which has many members and diverse functions. These small GTPases are not only essential for basic physiological activity, but also participate in many cellular processes and play a pivotal role in signal transduction regulation. According to our previously established expressed sequence tags (ESTs) library of Hevea latex, 6 full-length cDNAs encoding putative small GTPases were first cloned from the latex of Hevea brasiliensis by using reverse transcrip...
作者 石峰 秦云霞 唐朝荣
机构地区 中国热带农业科学院橡胶研究所 海南大学农学院
出处 《热带作物学报》 CSCD 2009年第3期320-326,共7页 Chinese Journal of Tropical Crops
基金 公益性科研院所基本科研业务费专项资金(NoXJSYWFZX2009-16) 973前期项目(No2008CB117009) 海南省自然科学基金项目(No808187)资助
关键词 巴西橡胶树 小G蛋白 全长CDNA 进化树 胶乳 Hevea brasiliensis small GTPase full-length cDNA phylogenetic tree latex.
分类号 S794.1 [农业科学—林木遗传育种]
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同被引文献52

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热带作物学报
2009年 第3期

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