山奈组织培养

Tissue Culture of Kaempferia galanga L

以山奈的带芽块茎为试验材料,研究其表面消毒的方法和不同配比培养基对山奈块茎诱导、增殖以及生根的影响。结果表明:外植体表面消毒采用体积分数75%酒精消毒1min,无菌水漂洗1次,然后用质量分数0.1%升汞浸泡10min,无菌水漂洗2次后,再用0.1%升汞浸泡6min,无菌水漂洗4次效果为最佳,污染率可降至6.67%;适宜的诱导培养基为MS+6-BA3.0mg/L+NAA0.1mg/L,芽的萌发率达100%,分化率达146.67%;增殖培养基以MS+6-BA3.0mg/L为最好,增殖倍数可达6.1倍;适宜的生根培养基为MS+NAA0.1mg/L,生根率可达100%;试管苗移栽到疏松透气的基质中即可,成活率可达93%。

Tubers carrying buds of Kaempferia galanga L. were used as explants for tissue culture, and different methods for superficial disinfection of the explants and different media for culture were tried to oberve their effects on bud shoot induction, proliferation and root growth of K. galangal L. The explants were superficially disinfected by soaking them 1 min with 75% alcohol, aseptically water rinsing 1 time, and then soaking them for 10 min with 0.1% HgCl2, aseptically water rinse 2 times, soaking them agai...