摘要
对Owayadei(Abelmoschus esculentus L.)、Ladies finger(Abelmoschus esculentus L.)、golder kost(Abelmosc hus esculentus L.)、Esoyarido(Abelmoschus esculentus L.)等42个国内外黄秋葵栽培种和野生黄葵(Abelmoschus moschatus Medicus Malv.)进行ISSR分析。结果表明,选用22条引物扩增出306条DNA片段,平均每个引物可扩增出13.9条片段,其中多态性片段271条,占扩增总片段的88.6%。利用扩增结果进行遗传距离分析,构建分子树状图,可把42份黄秋葵栽培种质和野生黄葵分开,同时,可将42份黄秋葵栽培种质材料划分为2个类群。
Forty-two accessions of okra germplasm, including Owayadei (Abelmoschus esculentus L.), Ladies finger (Abelmoschus esculentus L.), golden kost(Abelmoschus esculentus L.), Esoyarido (Abelmoschus esculentus L.) and wild Abelmoschus moschatus Medicus Malv. were analysed by using inter-simple sequence repeat (ISSR), and the results show that 306 fragments of DNA were amplified with 22 primers by using ISSR-PCR, among which 271 (88.6%) fragments were polymorphic. Each primer amplified 13.9 fragments of DNA. The ...
出处
《热带作物学报》
CSCD
2009年第3期293-298,共6页
Chinese Journal of Tropical Crops
基金
海南省自然科学基金项目(No30508)资助
