脂肪酶催化麦芽糖硬脂酸酯合成的研究

Lipase-catalyzed synthesis of maltose fatty acid ester

脂肪酸糖酯是一类用可再生资源合成的、无毒、易生物降解的非离子型表面活性剂,具有良好的乳化性、稠化性和膨化性,应用于食品、化妆品、制药和洗涤产品,是近20年来发展较快的一类乳化剂,以蔗糖酯为主,麦芽糖硬脂酸酯是一种新型的糖酯类乳化剂。本文利用固定化脂肪酶在有机溶剂中催化麦芽糖浆和硬脂酸的合成反应,研究了反应体系中脂肪酶的加入量、反应时间、反应温度、底物配比、体系含水量等因素对催化麦芽糖硬脂酸酯反应的影响规律,确定了酶促反应的工艺条件,同时对产物进行了定性、定量分析。研究结果表明:酶加入量为10.0%,反应温度75℃、含水量为有机溶剂的10.0%,底物中酸、糖的摩尔比为1:1,反应时间24h,在该条件下,酯化反应的最大转化率可达61.3%。

Sugar fatty acid ester is a kind of renewable synthetic resources.It is non-toxic,easy biodegradable,nonionic surfactant and has a good emulsifying property,viscous and swelling resistance.It is used in food,cosmetics,pharmacy and detergents.It is the fastest growing material over the last two decades.Maltose stearic acid ester is a new type of sugar ester emulsifiers synthesized by maltose syrup and stearic acid at the present of immobilized lipid enzyme as the catalyst.The dosage of lipase enzyme,reaction...