摘要
肌苷在食品和医药方面具有重要的研究意义,它可以治疗血小板减少症等病症和作为食品呈味剂的原料。肌苷生产菌在其整个生长过程中可以分为延滞期,指数期,稳定期和衰亡期四个阶段。通过对种子发酵液OD值的测定,可以确定菌体所处的生长时期的最佳时间,即处于对数生长期。OD值是光的吸收度,OD值越大,说明对光的吸收率也越大,菌体也越多。本实验主要通过L18(37)正交实验设计对发酵培养基中腺嘌呤、蛋白胨、氯化钠、尿素、玉米浆、葡萄糖、酵母膏等发酵条件进行优化,从而确定最佳发酵条件。试验结果显示:培养基为蛋白胨1.0%、腺嘌呤0.025%、葡萄糖2.2%、酵母膏1.4%、尿素0.8%、氯化钠0.25%、玉米浆1.5%,发酵8小时肌苷菌活性最好,肌苷产量最高。
Inosine has a important significant in the food and medicine because of its function on the treatment of thrombocytopenia.It is also a food flavor additive.Inosine are prepared by fermentation which have four stages:lag phase,exponential phase,stationary phases and decline phase in their entire growth process.The best time of bacteria growth is measured through to determine OD(optical density)of fermentation broth in the logarithmic phase.OD values are light absorbance,the bigger OD value,the large amount o...
出处
《中国食品添加剂》
CAS
北大核心
2009年第2期161-165,137,共6页
China Food Additives
关键词
腺嘌呤缺陷型
肌苷
优化条件
Adenine-deficient
inosine
optimize conditions
