摘要
在单因素试验基础上,首先用Plackett-Burman设计对影响荷叶总黄酮得率的因素进行了评价,筛选出具有显著效应的三个因素——乙醇浓度、液固比和提取次数;然后用响应面分析法确定了主要影响因素的最佳提取条件为乙醇浓度67%、液固比34:1(V/m)、提取3次,在此条件下荷叶总黄酮得率的预测值可达3.19%,实测值为3.17%,两者较接近。表明Plackett-Burman设计结合响应面分析法可以很好地对荷叶总黄酮提取工艺进行优化。
On the basis of single-factor tests, some factors affecting the extraction rate of total flavonoids from lotus leaves were firstly evaluated by Plackett-Burman design, and then the extraction conditions were optimized by response surface analysis. The results showed that the optimal extraction conditions are as follows: ethanol concentration 67%, ratio of ethanol solution to raw material 34∶1 and extraction times 3. Under the above conditions, the predicted value of extraction rate of total flavonoids is 3....
出处
《食品科学》
EI
CAS
CSCD
北大核心
2009年第6期29-33,共5页
Food Science
基金
教育部"长江学者和创新团队发展计划"项目(IRT0540)
江西省自然科学青年基金项目(2007GQN2012)
