三裂蟛蜞菊茎总黄酮含量的提取及其抗氧化性研究

Extraction and Antioxidant Activity of Total Flavonoids from Stem of Wedelia trilobata L.

本实验对从三裂蟛蜞菊茎中提取总黄酮的工艺进行优化,并对提取的总黄酮进行抗氧化性测定。结果表明:三裂蟛蜞菊的总黄酮含量为4.1%;最佳的工艺提取条件是70%的乙醇溶液按固液比1:25(m/V)配比,在温度50℃、功率210W超声波条件下处理20min;在本实验设置的三个质量浓度梯度条件下,总黄酮提取液对羟自由基、超氧阴离子自由基最大平均清除率分别达到20.16%、41.59%,对H2O2的最大抑制率达到55.22%,随着实验时间的增加,高质量浓度的提取液能显著延缓猪油自氧化的速度,虽然提取液具有一定的抗氧化能力,但与相应质量浓度的常用抗氧化剂BHT的抗氧化能力相比,仍存在显著的差异。

The extraction technology of total flavonoids from stem of Wedelia trilobata L. and its oxidation resistance were investigated. The results showed that the optimum extractant, liquid-solid ratio, temperature, ultrasonic wave power, ultrasonic wave treated time for extraction are 70% ethyl alcohol, 1:25, 50 ℃, 210 W and 20 min, respectively, and the obtained total flavonoids content from stem is 4.1%. Moreover, with three mass concentration gradients, the average maximum clearance rates of the extract to ·OH...