摘要
本实验以美国、阿根廷、巴西豆粉、中国豆粉和转基因大豆粉标准品为材料,利用设计的特异性引物和探针,建立了实时荧光定量PCR技术检测抗草甘膦转基因豆粉的方法,成功检测出美国、阿根廷、巴西抗草甘膦转基因豆粉和中国豆粉的转基因含量分别为5.37%、3.96%、2.26%和0,确定了荧光PCR技术检测抗草甘膦转基因豆粉的灵敏度为0.001%,稳定性良好。
A real-time fluorescence PCR method was developed for quantitative detection glyphosate-resistant genetically modified soybean powder. The detection results showed that the genetically modified (GM) contents of soybean powder from USA, Argentina, Brazil and China are 5.37%, 3.96%, 2.26% and 0, respectively. The sensitivity of established detection method is 0.001% and its stability is fairly good.
出处
《食品科学》
CAS
CSCD
北大核心
2009年第6期238-242,共5页
Food Science
基金
山东省教育厅资助项目(J06I55)
关键词
转基因豆粉
实时荧光PCR
检测
灵敏度
稳定性
genetically modified soybean powder
real-time fluorescence PCR
detection
sensitivity
stability
