DPH标记法研究自由基对活细胞膜流动性的影响

EFFECT OF FREE RADICALS ON INTACT CELL MEMBRANE FLUIDITY BY DPH LABELED

以组织培养的肝活细胞Ｌ－０２为材料，对荧光探剂ＤＰＨ标记活细胞膜脂的荧光光谱进行了研究，同时探讨了Ｆｅ（Ⅱ）诱发的活性氧自由基对人肝细胞膜脂流动性的影响及茶多酚的调节。结果表明，ＤＰＨ标记Ｌ－０２细胞后，荧光峰值蓝移，同时标记时间在３０－９０分钟间荧光偏振度P值基本稳定在 ０． ２７７－０． ２８０。 ０－５０ μ ｇ／ｍｌ的茶多酚对 Ｌ－０２细胞膜脂的流动性影响不大， Ｆｅ（Ⅱ）诱发的活性氧自由基则明显降低细胞膜的流动性， １０、 ２０、 ５０ μ ｓ／ｍｌ的 ＴＰ能保护细胞膜脂的流动性，且呈量效关系。

Taking hepatcyote lines L-02 as materials, the fluorescence spectrum of the fluorescent probe 1,6 dipheny-1,3,5 hexatriene(DPH) used to label the fluidity of intact plasma membranes is studied. Furthermore, influence on the damage of intact plasma membranes induced by free radicals and the regulation of tea polyphenols is researched. The result shows that the volue fluorescence peaks transfers from 442um to 432um, but the fluorescence Ploarization of cells membranes is stable betWeen 0.277 and 0.280 durin...