摘要
对猪生殖与呼吸综合征病毒(PRRSV)核衣壳蛋白(N)作为ELISA抗原进行了研究,将编码PRRSVN蛋白的基因0RF7cDNA插入杆状病毒表达载体pBlueBacHis2A,通过同源重组获得了重组杆状病毒ORF7-AcMNPV,感染昆虫细胞SF9表达了N蛋白,占细胞总蛋白的7.07%,纯化后作为抗原建立了间接ELISA,与IDEXX公司的ELISA诊断试剂盒有96%的符合率,与IFA有100%的符合率。试验证实:PRRSVN蛋白在昆虫细胞中得到高效表达且是检测PRRSV抗体的良好抗原。
A novel indirect ELISA using recombinant porcine reproductive and respiratory syndrome virus nucleocapsid protein as antigen was developed. The cDNA of PRRSV ORF7, which encodes nucleocapsid protein, was inserted into baculovirus expression vector pBlue- His2A. Recombanant baculoviruses were gained. After infection of SF9 cells, the expressed N protein could reach up to 7 .07% of the total cell proteins and can be detected by Westerblat, IFA. The affinity -purified protein was used as ELISA antigen. ...
出处
《中国预防兽医学报》
CAS
CSCD
2000年第S1期40-43,共4页
Chinese Journal of Preventive Veterinary Medicine