传染性法氏囊病RT-PCR诊断方法研究

Diagnosis of IBDV with RT-PCR

本文在研究传染性法氏囊病病毒的Ａ片断ｃＤＮＡ结构的基础上，用Ｐｒｉｍｅｒ Ｄｅｓｉｇｎ引物设计软件，在ＶＰ_５和ＶＰ_２的重叠基因区设计了一对引物，使用该引物对６种ＩＢＤＶ的标准毒株，１０例自然发病病鸡组织标本，８例ＩＢＤＶ Ｊ_１Ｃ_７Ｆ_３种毒株攻毒病理法氏囊组织标本，进行了ＲＴ－ＰＣＲ检测均得到了阳性的扩增结果。２种参考鸡病病毒和８例健康鸡的法氏囊组织在同样条件下进行扩增均为阴性结果。本法具有扩增方法简单，对各种病毒株的适应性广，特异性高，检测成本较低的特点。

After a carefully study on segment A of the cDNA of infectious bursal disease virus(IBDV), a pair of primers were designed with the Primer Design software.This primer pair is in the overlapping area of VP5 and VP2 where the sequence is highly homologous.6 stan- dard virus strains, 10 tissue specimen of natural diseased chickens and 8 pathology cloacial bursa specimen of IBDV J1C7F3 attacking test chickens had been amplified successfully with this pair of primers. 2 other comparison virus strains and 8...