反相高效液相色谱法测定人血中利福平浓度

Determination of rifampicin concentration in human serum by RP-HPLC

目的 :建立测定人血清中利福平浓度的高效液相色谱法 (HPLC)。方法 :血清加 4倍量的甲醇沉淀后离心 ,取上清液用 0 45 μ微孔滤膜滤过后进样。采用Nova-PakC1 8柱 ,以甲醇 -醋酸盐缓冲液 (62∶40 )为流动相 ,氯氮作为内标 ,在 3 3 4nm波长处测定。结果 :利福平的线性范围为 1～2 5 μg/ml,平均回收率为 (1 0 0 9± 4 2 ) % ,日内、日间RSD均小于 4%。最低检测限为 0 5 μg/ml(信噪比≥ 3 )。结论 :本法简便、可靠 ,适用于临床监测。

OBJECTIVE:To determine the concentration of rifampicin in human serum by RP-HPLC.METHODS:Serum was precipitated with centrifugation after add ed 4 times of methanol,and its super-stratum clear fluid was injected after filtration with 0.45μ micro-pore filter membrane.The method used Nova-Pak C 18 column as chromatogram column,methanol-acetate buffer solution as flow phase,chlordiazepoxide as internal standard.The detection wavelength was 334nm.RESULTS:The linear range of rifampicin was 1～25μg/ml.Average...