关附甲素对培养心肌细胞“缺氧缺糖”的保护作用

Effects of Guan-fu base A on neonatal rat myocytes in cultures with oxygen and glucose deprivation

目的 :研究关附甲素对培养心肌细胞“缺氧缺糖”的保护作用。方法 :以培养心肌细胞的“缺氧缺糖”模拟全体的缺血性损伤 ,以传统的钙离子拮抗剂———维拉帕未为对照药 ,观察关附甲素对“缺氧缺糖”心肌细胞乳酸脱氢酶 (LDH)生成 ,脂质过氧化代谢产物丙二醛 (MDA)的生成的影响。结果 :关附甲素 30ug/ml即能减少LDH的释放和MDA的生成 (P<0 0 5) ,且和维拉帕未有一定的相似性。结论 :关附甲素对“缺氧缺糖”心肌细胞具有一定的保护作用 ,且具有抗脂质过氧化作用 ,心肌细胞的保护作用可能和其抗脂质过氧化或抑制外钙内流有关。

OBJECTIVE:To investigate the effects of GFA on neonatal rat myocytes in cultures with oxygen and glucose deprivation(CMOGD).METHODS:By taking cultured neonatal rat myocytes with oxygen and glucose deprivation as ischemia-mimic condition of body and the Ca 2+ -antagonist Verapamil as control,the leakage of lactic dehydrogenase(LDH) and the production of malondialdehyde(MDA) were investigated. RESULT:GFA 30ug/ml suppessed the lactic dehydrogenase (LDH) leakage and the production of malondialdehyde (MDA) i...