黄孢原毛平革菌原生质体的制备与再生

STUDIES ON THE FORMATION AND REGENERATION OF PHANEROCHAETE CHRYSOSPORIUM

对黄孢原毛平革菌原生质体制备及其再生条件进行了较为详尽的研究和报道 ,结果表明使用萌发的分生孢子作为制备原生质体的细胞来源 ,以 0 .6mol/LMgSO作为渗透压稳定剂 ,利用 10mg/mLNovozyme 2 34和溶壁酶共同消化分生孢子 ,可获得 85%以上的原生质体形成率 .原生质体在CMS培养基中再生率为 10 % ,在Gold’sBI培养基中为 2 %～6 .5% .

The condition of protoplast formation and regeneration of P.chrysosporiun were reported. The results indicated that used 0.6mol/L MgSO 4 as osmotic stabilizer, both Novozyme 234 and Lywallzyme were required to digest spores together, the yield of protoplasts is above 85%; The regeneration frequency is 10%～12% in CMS medium, and 2%～6.5% in Gold’s BI medium.