一种新型腺病毒突变体的抗肿瘤实验研究

EXPERIMENTAL STUDIES ON A NOVEL ANTI-TUMOR ADENOVIRUS MUTANNT

研究观察了腺病毒突变体dl152 0诱导 p53缺陷的肿瘤细胞的凋亡作用和对荷瘤小鼠的抗瘤效应 .结果表明 ,腺病毒突变体dl152 0感染具有 p53基因突变或缺失的肿瘤细胞后 ,能引起肿瘤细胞的凋亡 ;而对具野生型 p53的肿瘤细胞 ,则基本上不导致肿瘤细胞的凋亡 .同时细胞周期分析表明dl152 0使p53缺陷肿瘤细胞的细胞周期明显改变并阻止于G1期 .荷瘤小鼠实验表明 ,荷瘤小鼠在注射腺病毒突变体dl152 0后 ,致使肉瘤绝大部分坏死 ,并持续萎缩 ,直至最终完全消退 .上述结果提示腺病毒突变体dl152 0在治疗人的 p53缺陷肿瘤方面有着良好的应用前景 .

In the present study, the apoptosis of p53 deficient tumor cells induced by adenovirus mutant dl1520 and the antitumor effects of dl1520 on tumor bearing BALB/c mice were observed. The results showed that dl1520 induced apoptosis of the human tumor cells of deletion or mutation of p53 tumor supressor gene after it infected, however,it didn’t induce apoptosis of the human tumor cells with .functional p53. The cell cycle analysis showed dl1...