乙肝病毒(adr亚型)X基因转基因小鼠的建立

THE ESTABLISHMENT OF TRANSGENIC MICE OF HEPATITIS B VIRUS X GENE(ADR SUBTYPE)

运用二种制备转基因动物方法———显微注射法和以精原干细胞转染法 ,生产乙肝病毒X基因转基因小鼠 .再由PCR和肝组织免疫组化法 ,检测用上述两种方法生产的小鼠 .

HBV only infects human being and primates in natural condition, which bring more difficult for the study on HBV. The establishment of technique of transgenic animal model of HBx brings about a new experimental system for this field. Establishment transgenic mice of HBV X gene was carried out by following two methods: (1) Linearized pcDNA3 X was injected into the male pronuclei of fertilized eggs by microinjection, then transferred to ...