溶栓治疗对急性心肌梗死患者中性粒细胞表面补体含量的影响

The influence of fibrinolytic therapy on the deposition of complement segment on circulating neutrophils in patients with acute myocardial infarction

目的 探讨静脉溶栓治疗对急性心肌梗死患者血液中性粒细胞表面的补体含量的影响。方法 用免疫荧光标记法和流式细胞术 ,测定 16例急性心肌梗死患者静脉溶栓后沉积于中性粒细胞表面的补体片段C3c含量的动态变化。结果 溶栓后 30min ,中性粒细胞表面的C3c含量升高 3倍 ,溶栓后 6 0min和 12 0min恢复至溶栓前的水平。结论 溶栓治疗激活急性心肌梗死患者的补体系统 ,使沉积于中性粒细胞表面的补体片段含量一过性升高。这些变化是否对急性心肌梗死的病理过程产生不利的影响 ,值得进一步探讨。

Objective This study was designed to evaluate the influence of intravenous fibrinolytic therapy on the deposition of complement segment on circulating neutrophils in patients with acute myocardial infarction. Methods After intravenous fibrinolytic therapy in 16 patients with acute myocardial infarction, the serial changes of the deposition of C3c segment on the surface of circulating neutrophils were investigated by immunofluorescence and flow cytometry. Results The deposition of C3c on neutrophils increased three times 30 minutes after fibrinolysis and returned to baseline 60 minutes and 120 minutes after fibrinolysis. Conclusion Fibrinolytic therapy activated the complement system and resulted in a transient increase of the deposition of complement segment on circulating neutrophils, which might make an adverse influence on the pathological process of acute myocardial infarction.