^(14)C-丁草胺和^(14)C-毒死蜱在鲮鱼Cirrhina　molitorella中的积累和代谢

Accumulation and Metabolism of ^(14)C-Butachlor and ^(14)C-Chlorpyrifos in Cirrhina Molitorella

鲮鱼 Ｃｉｒｒｈｉｎａ ｍｏｌｉｔｏｒｅｌｌａ能迅速积累水中的～（１４）Ｃ－丁草胺和～（１４）Ｃ－毒死蜱并在达到峰值后明显下降．在８ｄ时丁草胺组的鲮鱼各器官组织放射比活度高低次序为：内脏团＞鳍＞整体＞眼＞鳃＞肌肉＞剩余部分，分别为其峰值的２０．４％、２７．１％、１１．６％、３９．７％、１０．３％、９．４％和６．２％．在毒死蜱组，鳍的放射性比活度峰值出现在２ ｈ，其余器官的在１２ ｈ～１ｄ之间出现，各器官组织的峰值高低顺序为：内脏团＞整体＞鳍＞眼＞剩余部分＞鳃＞肌肉；在第８天时各对应器官组织的比活度分别为其峰值的１１．５％、１２．６％、１７．３％、１３．６％、１０．７％、１７．５％和１０．４％．共层析法研究表明了草胺很快被降解为ＤＡ等极性较大的物质，母体丁草胺的比例很低；相对于丁草胺，毒死蜱较难降解，其代谢物以ＴＣＰ为主．鲮鱼中的丁草胺的结合态为１９．１％～６４．５％，毒死蜱的结合态为１４．４％～７２．８％．

Mud carp Cirrhina molitorella rapid accumulated ^(14)C-butaclilor and ^(14)C-chlorpyrifos in wa- ter, with greatly different ability between the organs. After the accumulation peaks, the radioactivity decreased obviously. The radioactivity of ^(14)C-butachlor in descendant order on 8d was: viscera, fin, the whole body, eye, gill, muscle, and the residue tissues, which were very low compared to their peaks, just2O.4%, 27.1%, 11.6%, 39.7%, 10.3%, 9.4%, and6.2% of their peaks, respec- tively. The radioactivity of ^(14)C-chlorpyrifos in fin reached the peak at 2h. The other organs reached their accumulation peaks of ^(14)C-chloipyrifos at 12 h or on 1 d, with the radioactivity in descendant or- der of: viscera, the whole body, fin, eye, the residue tissues, gill, and muscle. After the peaks, the radioactivity on 8 d decreased to just 11.5%, 12.6%, 17.3%, 13.6%, 10.7%, 17.5%, and 10.4% of their own peak values, relevantly to the ordered organs/tissues. Co-chromatography analysis demonstrated that butachlor was rapidly degraded into polar compounds in both viscera and the residue tissues. The parent butachlor was very little in the metabolites after 6 h of the experiment. Chlorpyrifos was difficult to be rapidly degraded in viscera of mud carp with TCP and the polar compounds as the major metabolites. Moreover, 19.1 ~ 64.5% of ^(14)C-butachlor and 14.4 ~ 72.8% of chlorpyrifos in mud carp was bound to the biological tissues during the experiment.