人嗜酸乳杆菌对幽门螺杆菌的体外拮抗作用

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摘要目的探讨人嗜酸乳杆菌对幽门螺杆菌(Helicobacterpylori,Hp)毒力株的体外拮抗作用,筛选对Hp毒力株有明显拮抗作用的嗜酸乳杆菌菌株.方法从健康人胃肠道分离出52株嗜酸乳杆菌可疑株,经培养特性,生理特性,生化反应及代谢产物等鉴定,获得26株嗜酸乳杆菌.并从患者胃活检标本中分离出23株Hp,用PCR方法筛选出cagA阳性Hp毒力株.采用打孔法进行嗜酸乳杆菌拮抗Hp毒力株的实验:取0.1mLHp菌液于灭菌平板中央,倾注冷至约40℃培养基,待凝固后打孔,加满嗜酸乳杆菌培养上清,37℃微需氧培养72h,观察抑菌圈大小,以MRS肉汤、1%乳酸对照.还测定嗜酸乳杆菌培养上清和1%乳酸的pH值.结果筛选出4株对Hp毒力株有明显拮抗作用的嗜酸乳杆菌,应用t检验,将嗜酸乳杆菌上清组抑菌圈直径与1%乳酸对照组比较,P<0.001,有显著性差异.嗜酸乳杆菌培养上清液的pH值高于1%乳酸,表明嗜酸乳杆菌对Hp的拮抗作用是乳酸非依赖性的.结论人嗜酸乳杆菌在体外对Hp毒力株具有明显拮抗作用.该研究为应用微生态疗法治疗Hp感染提供了理论基础.

作者龙敏龙北国别平华罗军

机构地区中国人民解放军第一军医大学微生物教研室广东省产州市

出处《世界华人消化杂志》 CAS 2000年第z1期38-,共1页 World Chinese Journal of Digestology

基金全军"九五”青年基金课题

关键词螺杜菌幽门嗜酸乳杆菌毒力体外发生活组织检查聚合酶链反应

分类号 R573 [医药卫生—消化系统]

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人嗜酸乳杆菌对幽门螺杆菌的体外拮抗作用

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