新生兔颅骨改良组织块移行法成骨细胞的体外培养

Osteoblast isolated and cultured in vitro by emigrated from nowborn rabbit cranium.

目的 建立一种成骨细胞体外培养模型。方法 以新生兔颅骨为材料 ,用改良组织块移行生长法在体外培养获得贴壁生长的细胞 ,并对其形态 ,生长特点及生物学特性进行研究。结果 获得的细胞呈多种生长形态 ,如梭形 ;三角形 ;鳞片形 ;星形等 ,有较多的细胞突起 ,细胞呈复层生长 ,互相重叠 ,形成细胞结节 ,碱性磷酸酶(AL P)染色强阳性 ,在含有 Vit C和 β—甘油磷酸钠的培养条件中出现钙化现象。结论 采用改良组织块移行生长法获得的细胞具有成骨细胞的特性 ,该方法是体外培养、研究成骨细胞的良好模型。

Objective To establish a mode of osteoblasts culture in vitro. Methods Osteoblasts were cultured and isolated by emigrated from nowborn rabbit cranium in vitro, and study it's biological characterization. the cells were examined by phase-contrast microscope, traansmission electron microscope, scanning electron microscope, histochemistry stains and calvaria strains.Results Osteoblasts were increased, cube-shaped, and gathered togather, The variation of their appearance was observed, had the same morphological feature, ALP activity and mineralized matrix fromed in vitro.Conclusion It is a good method of osteoblasts culture in vitro that osteoblasts isolated by emigrated.