稻瘟病菌毒素对水稻根冠细胞微丝的影响

Effect of Pyricularia Oryzae Toxins on Microfilament of Rice Root Cap Cells

用稻瘟病菌 90 - 2菌株产生的毒素 ,处理水稻同核异质野败型雄性不育细胞质珍汕 97A及其保持系正常细胞质珍汕 97B的根冠细胞 ,以异硫氰酸荧光素 -鬼笔环肽为探针 ,观察了毒素处理前后细胞微丝骨架的形态及分布变化。结果显示 ,毒素处理前珍汕 97A ,珍汕 97B的根冠细胞中 ,微丝由内向外广泛散布 ,粗细不等 ,呈无序网络分布。毒素处理后 ,珍汕 97A的根冠细胞微丝的密集分布被破坏 ,纤丝状微丝不复存在 ,微丝集聚成粗束 ,有的呈碎片状 ;而珍汕97B的根冠细胞微丝破坏程度轻 ,微丝分布类似于毒素处理前的分布。本文研究了在毒素作用下 ,不同细胞质微丝状态及分布的变化 。

The distribution of actin filaments (AFs) in the root cap cells of rice Zhenshan 97A and Zhenshan 97B was studied using fluorescence probe FITC-Phalloid in. When the root cap cells were treated by toxins,the distrbution pattern of AF s was changed into bundles or granules. The AFs in Zhenshan 97A were destroyed mo re than that of in Zhenshan 97B. As a extracelluar signal,the toxins have the pa thogenicity to the rice by some way. The results suggest that the microfilment is related to th e signal transduction.