小鼠肝癌抗原粗提物致敏树突状细胞体外诱导特异性细胞毒T淋巴细胞的研究

In vitro INDUCTION OF ANTI-TUMOR RESPONSIVENESS BY DENDRITIC CELLS PULSED WITH TUMOR EXTRACTS

目的 探讨小鼠骨髓树突状细胞（ｄｅｎｄｒｉｔｉｃ ｃｅｌｌ，ＤＣｓ），体外经Ｈｅｐａ１－６肝癌细胞株抗原粗提物致敏后，对特异性细胞毒 Ｔ淋巳细胞（ＣＴＬ）的诱导作用。方法 自小鼠骨髓分离 ＤＣｓ前体细胞，经 ＧＭ－ＣＳＦ＋ＩＬ－４培养、扩增；制备 Ｈｅｐａ １－６小鼠肝癌细胞株抗原粗提物，体外脉冲ＤＣｓ，检测ＤＣｓ诱导同基因型小鼠Ｔ细胞增殖能力及特异性ＣＴＬ的反应能力。结累和结论 经肿瘤抗原致敏的ＤＣｓ组织相容性分子（ＭＨＣ－Ⅰ、Ⅱ）及共刺激分子（Ｂ７－２、Ｂ７－１）表达明显增高，能诱导较强的同基因型小鼠Ｔ细胞增殖．且能诱导Ｈｅｐａ １－６特异性细胞毒Ｔ淋巴细胞。

Objective The present stud y was designed to inv estigate whether pulsing DCs with tumor-derived extracts is an effective way to induce CTL and antitumor immunity.Methods DCs were propagated f r om bone marrow (BM)of C57BL/6J(H-2Kb、I-Ab)mice in vitro with GM -CSF+IL- 4tumor associated antigen (TAA)extracted from actively growing Hepa 1-6 cells w as used to activate DCs.The phenotypes of DCs were detected by FACS,the cytotoxi c ity of CTL was assayed by 3H-TdR labbel assay.Result and Conclusi on The TAA extract pulsed DCs exhibited much more and longer cell processe s and increased expression of MHC-I,MHC-II,CD80(B7-1)、CD86(B7-2).This expe riment has shown that DCs pulsed with TAA extracts of C57B/6J cells could stimul ate effectively the responsiveness of syngenic splenic T cells to induce specifi c CTL against C57BL/6J cells.