芳香化酶在神经干细胞及其早期分化中的表达

EXPRESSION OF AROMATASE CYCOCHROME P - 450 IN THE NEURAL STEM CELLS AND THE EARLY DIFFERENTIATED CELLS

目的 研究芳香化酶在神经干细胞及其分化后的基因表达。方法 神经干细胞培养，免疫细胞化学及ＲＴ－ＰＣＲ技术。结果 神经干细胞内有芳香化酶的基因表达，其分化后神经元中芳香化酶强表达而神经胶质细胞中弱表达；这些细胞中芳香化酶的表达主要受脑组织特异启动子１．４驱动。结论 可能为中枢神经系统内雌激素的来源提供新的线索。

Objective To explore the gene expression in the neur al stem cells as well as the cells after the clone was differentiated. Methods Neural stem cell culture,Immunocytochemistry and RT-PCR techniq ue were used.Results There was Aromatase expression in the neur al stem cells,after the stem cells were differentiated,the Aromatase strongly expre s sed in the neruons and weakly expressed in the astrocytes.The aromatase gene in these cells was expressed by means of the brain-specific promoter 1 .4 .Conclusion It may provid a new clue for the source of estrogen in the central nerve system.