膀胱移行上皮细胞癌患者手术前后外周血双微体的表达意义

DOUBLE MINUTES EXPRESSION AND ITS SIGNIFICANCE IN PREOPERATIVE AND POSTOPERATIVE PATIENTS WITH BLADDER TRANSITIOAL CELL CARCINOMA

目的 研究双微体（ｄｏｕｂｌｅ ｍｉｎｕｔｅｓ，ＤＭ）在膀胱移行上皮细胞癌中的表达及其意义。方法 正常对照组 １５例，膀胱移行上皮细胞癌术前术后组各１４例，采用外周静脉血全血培养，常规染色体准备，检测ＤＭ的表达，采用统计学ｘ２检验。对ＤＭ的表达与临床病理因素及预后的关系进行分析。结果 正常对照组无ＤＭ检出，术前术后组分别有３５和 ３１对ＤＭ检出，与对照有显著性差别（Ｐ＜０．０１），且集中于两例ＤＭ阳性病例，但术前术后无显著性差别（Ｐ＜０．０５）。结论ＤＭ可作为癌基因扩增的一项独立的预后指标，ＤＭ阳性患者，预后差，易复发。

Objective To investigate the expression of doubl e minutes (DM)in bladder transitional cell carcinoma (TCC)and its significance . Methods 15 control individuals and 14 patients were examined . Whole blood samples from these patients were cultured before and 10-15 days aft e r operations.The relationship between expression of DM and clinic-pathologic fa ctors and prognosis was analyzed by chi-square test.Results T here is no DM in control individuals,but there are respectively 35 pairs and 31 pairs DM in preoperative and postoperative patients with TCC (P＜0.01)and concen trate in two DM (+)patients.However,the difference between preoperative and post operative patients was not statistically significant(P＞0. 05).Conclus ions DM may be an independent prognosis indication of cancer gene amplic ation.DM always show treatment failure and rapid recurrence.