摘要
目的 研究二氢毗陡类钙桔抗剂，［２，６ 二甲基－４－（３－硝基苯基）－１，４－二氢－３，５－吡啶二羧酸－３－甲酯－５－正戊酯（Ｍｎ９２０２）了、拉西地平（ｌａｃｉｄｉｐｉｎｅ）对培养的大鼠胃底平滑肌 ５－ＨＴ、５－ＨＴ合成酶（ＴＨ）及 ５ＨＴ受体（５－ＨＴＲ）的影响。方法采用免疫细胞化学和图像分析等方法观察Ｍｎ９２０２和ｌａｃｉｄｉｐｉｎｅ作用下培养的大鼠胃底平滑肌细胞中５－ＨＴ、ＴＨ和５－ＨＴＲ含量的变化．并与５－ＨＴ的桔抗剂赛庚定（ｃｙｐｒｏｈｅｔａｄｉｎｅ）比较。结果 正常大鼠胃底平滑肌细胞５－ＨＴ、ＴＨ、５－ＨＴＲ免疫反应均呈阳性，而Ｍｎ９２０２、ｌａｃｉｄｐｉｎｅ和ｃｙｐｒｏｈｅｔａｄｉｎｅ均能使胃底平滑肌５－ＨＴ、ＴＨ和５－ＨＴＲ的含量减少。结论 大鼠胃底平滑肌胞具有自分泌５－ＨＴ的功能，Ｍｎ９２０２和拉西地平可能通过减少５－ＨＴ的合成而使５－ＨＴＲ的表达下降，这一结果与５－ＨＴ桔抗剂赛庚定相似。
Objective To study the e ffects of DHPs calcium antagonist Mn9202 and lacidipine on 5-HT、TH and 5-HTR of rat stomach fundus smooth muscle cells.Methods It's effects was investigated by using immun ocytochemistry and image analysis of stomach fundus smooth muscle cells in culture and compared with cyprohetadine(antagonist of 5-HT).Results 5-HT、TH and 5-HTR immunoreactive substances were existed in SFSMC.The results of image analysis indicated that the content of 5-HT、TH and 5-HTR were significantly lower when using Mn9202、lacidipine and cyprohetadine.Conclusion The results sugg est that the rat stomach fundus smooth muscle cells possess 5-HT autocrine function,and DHPs calcium antagonist reduce the number of 5-HT receptor in the rat stomach fundus smooth muscle cells though the reduction of 5-HT sythesis,this results conform with cyprohetadine(antagonist of 5-HT）.
出处
《解剖学报》
CAS
CSCD
北大核心
2000年第z1期126-128,148,共4页
Acta Anatomica Sinica
