下颌骨种植体周围炎模型建立的动物实验研究

Experimental research about animal models of per-implantitis

目的:在兔颌骨无牙区植入的种植体上使用丝线栓结法建立种植体周围炎模型。方法:6只成年兔,在双侧无牙区均植入2mm直径一段式羟基磷灰石涂层种植体,骨结合3个月后,一侧栓丝引入炎症,另一侧做为对照。在栓丝后第4,8,12周分别探查其炎症程度,第6及12周拍摄X线进行观察。同时在第12周时处死动物进行组织学观察,观察种植体周围的骨缺损程度及炎症程度并与对照组进行对比研究。结果:结果表明,对实验组的菌斑指数(PI)、牙龈指数(GI)、探诊深度(PD)进行探查均显示有明显炎症,栓丝3个月后的兔下颌骨标本种植体周围缺损深度(DD)、缺损宽度(DW)存在。X线显示栓丝后3个月的骨密度明显降低。甲苯胺蓝染色片显示实验组缺损区骨组织缺如,部分纤维组织存在。相对而言,对照组未探及明显炎症,X线显示骨结合良好,甲苯胺蓝染色显示种植体-骨结合界面良好。结论:在兔颌骨上无牙区植入种植体后,使用丝线栓结法能成功建立种植体周围炎的模型。

Objectives:Success to establish peri-implantitis models on rabbit mandible by ligature-induced.Methods:6 adult rabbit would receive 2mm diameter hydroxyapatite-coated implants implanted in the toothless areas.After 3 months osseointegration,silk ligatures were placed around one side of the implants to induce peri-implantitis,the other side as a control.After ligature-induced,examine its inflammation in the 4、8、12 weeks,take X-rays in the 6、12 weeks.At the same time in the 12 weeks when the animals were killed for morphological observation.Contrast the control group,observe the bone defect around the implants and the degree of inflammation.Result:The results showed that the experimental group on the plaque index(PI),gingival index(GI),probing depth(PD)have detect obvious inflammation.3months after ligature-induced,defect depth(DD),defect width(DW)exist on the the samples of rabbit mandibular.And X-rays showed bone mineral density decreased significantly.Morphological observation showed the area of experimental group has bone losses,part of them has fibrous tissue existence.Compare to the experimental group,inflammation did not be probed on the control group.x-ray andmorphological observation showed good osseointegration interface Conclusion:Peri-implantitis models can be successfully established on the toothless implant of the mandible of the rabbit by ligature-induced method.