三氯乙烯所致接触性皮炎小鼠免疫功能的变化

Immune function changes caused by contact dermatitis in mice after intracutaneous injection of trichloroethylene

目的研究三氯乙烯(TCE)刺激BALB/c小鼠产生接触性皮炎,观察TCE对小鼠免疫功能的影响。方法采用背部皮内注射TCE染毒BALB/c小鼠,观察脾脏、胸腺脏器系数的变化情况,对脾和胸腺细胞进行体外淋巴细胞活力和增殖功能测定,腹腔巨噬细胞吞噬功能测定,NK细胞杀伤活性测定及小鼠血清中肿瘤坏死因子(TNF-α)、干扰素(IFN-γ)含量的测定。结果TCE实验组胸腺脏器系数显著低于阴性对照组(P<0.01);TCE实验组T淋巴细胞活力及增殖能力显著高于阴性对照组(P<0.01);TCE实验组的巨噬细胞吞噬率和NK细胞杀伤活性显著高于阴性对照组(P<0.01);TCE实验组小鼠血清中TNF-α的含量显著高于阴性对照组(P<0.01)。结论三氯乙烯能够诱发接触性皮炎,其中毒机制属于迟发型超敏反应。

Objective To investigate the contact dermatitis of BALB / c mice exposed to Trichloroethylene (TCE) via back intradermal injection and observe the influence on immune function of BALB/c mice. Methods BALB /c mice were exposed to TCE through intradermal injection. The organ coefficient of spleen and thymus were calculated. Lymphocytes viability and lymphocytes proliferation assay, Macrophage activation and NK cell activity assay were carried out; the content of TNF-α and IFN-γ in the serum were measured. Results The thymic organ coefficient of TCE group was significantly lower than that of the negative control group (P<0.01). The lymphocytes viability and lymphocytes proliferation of TCE group were obviously higher than those of the negative control group (P<0.01). Compared to negative control group, the response of macrophage activation and NK cell activiy of the TCE group were enhanced markedly (P<0.01). in TCE group the serous content of TNF-α was evidently higher than that of the negative control group (P<0.01). Conclusion Trichloroethylene as an allergen can cause contact dermatitis whose mechanism was supposed to be a delayed-type hypersensitivity.