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人成纤维细胞生长因子-21分泌型表达及鉴定 被引量：9

Identification and Expression of Secretary Human Fibroblast Growth Factor(FGF)21
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摘要 利用基因工程手段,将人成纤维细胞生长因子-21(hFGF21)与分泌信号肽相融合,构建得到能够分泌表达FGF21蛋白的表达载体。将该表达载体导入到Rosetta(blue)宿主细胞中,在分泌信号肽的引导下,在细胞周质中表达。经IPTG诱导表达可获得分子量为21 kD的蛋白,Western-Blotting证明该蛋白为FGF21蛋白。经SDS-PAGE证明获得了可溶性的FGF21蛋白。该方法简化了纯化工序,提高了成品率,使大规模生产重组人成纤维细胞生长因子-21(rhFGF21)成为可能。 FGF21 was cloned into the expression vector containing signal peptide gene,then transformed into Rosetta(blue) cell and the transformant was selected by ampicillin resistance.Under the signal peptide′s guidance,FGF21 was expressed in the cell periplasmic space.A protein with molecular weight of about 21 kD was induced by IPTG.Western blotting analysis showed that the protein was FGF21.After SDS-PAGE analysis,the protein of secretary hFGF21 was obtained which indicated that the purification procedure of this protein would be very simple. As a result,the rate of products would also be increased and the large scale production of rhFGF21 became possible.
作者 王会岩 张耀方 万晓珊 解佳森 肖业臣 李校堃
机构地区 吉林农业大学生物反应器与药物开发教育部工程研究中心
出处 《吉林农业大学学报》 CAS CSCD 北大核心 2009年第3期255-257,272,共4页 Journal of Jilin Agricultural University
基金 吉林省科技发展计划项目(20080712) 长春净月科技三项费用项目(2008B003) 吉林农业大学博士启动基金项目(2007013)
关键词 人成纤维细胞生长因子-21 pET-22b 分泌表达 hFGF21 pET-22b secretion expression
分类号 Q786 [生物学—分子生物学]
  • 相关文献

参考文献10

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共引文献42

同被引文献85

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引证文献9

二级引证文献18

吉林农业大学学报
2009年 第3期

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