鲫鱼体内超氧化物歧化酶对外源性MTBE胁迫的响应

Response of Activities of SOD in Carassius auratus to MTBE Stress

通过染毒试验,研究不同质量浓度外源性甲基叔丁基醚(MTBE)对鲫鱼鳃、肝胰脏、肌肉中超氧化物歧化酶(SOD)活性的影响。结果表明:不同MTBE质量浓度和胁迫时间条件下,鲫鱼鳃、肝胰脏和肌肉中SOD活性受到不同程度的影响。胁迫48 h时,鲫鱼肌肉和肝胰脏的SOD活性显著高于胁迫96,144 h;胁迫96 h时,鳃中SOD活性最高。在相同暴露条件下,鲫鱼肌肉中SOD活性与鳃中相近,但明显低于肝胰脏中(P<0.05)。短时间(48 h)胁迫时,MTBE胁迫对鲫鱼肌肉和肝胰脏SOD的活性起诱导作用,长时间胁迫则有一定的抑制作用。96 h时MTBE胁迫对鲫鱼鳃中SOD活性起诱导作用。鲫鱼体内SOD活性的变化可以间接地反映水体MTBE的污染程度,可作为早期检测MTBE对水环境污染的生物标记。

Effects of MTBE on activities of superoxide dismutase in gills,livers,and muscles of Carassius auratus exposed to different concentrations of MTBE were studied.The results show that effects of MTBE on activities of SOD in gills,livers and muscles of Carassius auratus varied with the concentration of MTBE and exposure time;activities of SOD in muscles and livers at 48 h were higher than those at 96 h and 144 h after the exposure.But for the activities of SOD in gills,the maximum was at 96 h.Under the same exposing conditions,activities of SOD in gills were lower than those in livers(P<0.05),but closer to those in muscles.At the same exposure time,lower concentration of MTBE induced the activities of SOD,but higher concentration of MTBE inhibited them.Change of SOD activities in Carassius auratus can indirectly affect the degree of pollution by MTBE in water body,which can be used as one bio-marker in testing water pollution with MTBE.