SARS患者康复后血清抗SARS病毒特异性抗体IgM和IgG动态变化

Dynamics of SARS Coronavirus Specific Antibodies IgM and IgG in Patients with SARS

目的:探讨SARS患者康复后4年内血清抗SARS病毒特异性抗体IgM(SARS-CoV-IgM)和IgG(SARS-CoV-IgG)的动态变化。方法:前瞻性跟踪50例SARS患者血清抗SARS-CoV-IgM和IgG的变化。用ELISA法检测该组患者患SARS后第0～10 d、11～20 d、21～30 d、31～40 d、41～50 d、51～60 d、61～70 d、71～80 d、81～90 d、91～120 d、121～150 d和第4年血清抗SARS-CoV-IgM和IgG的变化。结果:抗-SARS-CoV-IgM和IgG阳性率均在SARS发病10d后逐渐升高,并在发病后31～40d达100%,但抗-SARS-CoV-IgM阳性率在发病51～60d后逐渐下降,发病后第4年为0:抗SARS-CoV-IgG阳性率在发病81～90d后逐渐下降,发病后第4年为47%。在体内维持时间较短,而IgG下降缓慢,在体内维持时间较长。结论:根据SARS患者血清中抗-SARS-CoV-IgM在体内维持时间短和IgG在体内维持时间长的特点,抗-SARS-CoV抗体可用于SARS流行病学追踪调查。

Objective:To survey the dynamics of Severe Acute Respiratory Syndrome Coronavirus(SARS-CoV) IgM and IgG Antibodies in Patients with SARS.Methods:50 patients with SARS were prospectively studied.The serum samples of the cases were collected on 0～10 d、11～20 d、21～30 d、31～40 d、41～50 d、51～60 d、61～70 d、71～80 d、81～90 d、91～120 d、121～150 d and 4th year after the onset of the disease.The titer of SARS-CoV specific IgM and IgG antibodies were tested by enzyme-linked-immunosorbent assay.Results:The positive rate of SARS-CoV antibodies was low within 10d after the the onset of SARS.They reached a peak 31～40d after,and then they gradually came down.The positive rate of SARS-CoV-IgM decreased 51～60 d after the the onset and reduced to zero 4 years after.The positive rate of SARS-CoV-IgG decreased 81～90d after the the onset and reached 47%4 years after.Conclusion:The SARS-CoV antibodies in Patients with SARS could be use in epidemiology accoding to their dynamics that anti-SARS-CoV IgM appeared earlier and disappeared more quickly than anti-SARS-CoV IgG.