哮喘患者外周血IL-16水平及其多态性检测

Detection of Peripheral Blood Interleukin-16 and Its Gene Polymorphism in Asthma Patients

目的探讨哮喘患者外周血IL-16的变化及IL-16基因多态性(T-295C)与哮喘的关系。方法选择确诊的42例哮喘患者为哮喘组,并选择同期健康体检者37例为对照组,应用酶联免疫吸附试验(ELISA)双抗体夹心法检测哮喘患者外周血IL-16水平,应用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)方法检测IL-16基因多态性,并进行统计学分析。结果哮喘组血浆IL-16水平为(89.4±8.2)ng/L,与对照组(30.8±5.2)ng/L比较差异有统计学意义(P<0.05);哮喘组和对照组IL-16基因259位点基因型分布及等位基因突变频率间差异均无统计学意义(P>0.05)。结论IL-16参与了哮喘发病的病理生理过程,但IL-16突变与哮喘无相关性。

Objective To investigate the association of change of interleukin-16(IL-16) of peripheral blood and its gene polymorphisms with asthma.Methods Totally 42 diagnosed asthmatic patients were enrolled as the subjective,concurrently with 37 health persons as the controls.The serum level of IL-16 was measured by double antibody sandwich method of ELISA.The plasma IL-16 genotypes were determined by PCR-RFLP.Results The level of serum IL-16 in asthmatics((89.4±8.2)pg/ml) was higher than that of the controls((30.8±5.2)pg/ml)(P<0.05).The genotype distribution and allele mutation frequencies of IL-16 of 259-gene locus in asthmatics showed no difference from those in the controls(P>0.05).Conclusion IL-l 6 takes part in path physiological process of asthmatic pathogenesis.However IL-16 gene mutation is not related with asthma.