秋花独蒜兰原球茎液体快速增殖和分化培养研究

Rapid PLBs proliferation and regeneration in liquid culture of Pleione maculate Lindl.

采用液体振荡培养的方法进行秋花独蒜兰原球茎的快速增殖和分化试验,分别研究了不同基本培养基、激素种类及其浓度、不同天然添加物对原球茎增殖的影响,在此基础上进行了原球茎快速分化的研究。实验结果表明,秋花独蒜兰原球茎的快速增殖培养基为:花宝0.3%+糖2%+BA0.5 mg/L;而液体→固体培养基形式的分化培养比固体→固体培养基形式的分化速度快,且分化的芽的粗壮程度高于固体→固体培养基形式中的芽。

The protocorm like bodies of Pleione maculata Lindl. were used as explants for the rapid proliferation and regeneration in liquid culture with different media,organic matters and different concentration of hormones. The results showed that the best medium for proliferation of P. maculata PLBs was hypnex 0.3%+sucrose 2%+BA0.5 mg/L. The rate of PLBs regeneration in the culture style of liquid to solid medium was significantly higher than that in the culture style of solid to solid medium, and the plantlets in the culture style of liquid to solid medium were much stronger.