我国旋毛虫地理株的同工酶分析

Isoenzymes analysis of Trichinella isolates from China

目的为旋毛虫属的分类提供依据。方法应用聚丙烯酰胺凝胶电泳(PAGE)对我国6个猪源旋毛虫地理株(河南、云南、哈尔滨、黑龙江同江、湖北及天津)肌幼虫的超氧化物歧化酶(SOD)、苹果酸酶(ME)、酯酶(EST)、谷氨酸脱氢酶(GLDH)进行分析,并以旋毛虫(Trichinella spiralis,T1)、乡土旋毛虫(T.nativa,T2)、布氏旋毛虫(T.britovi,T3)、伪旋毛虫(T.pseudospiralis,T4)及纳氏旋毛虫(T.nelsoni,T7)的国际参考株作为对照。结果我国6个猪源旋毛虫地理株的4种同工酶(SOD、ME、EST、GLDH)酶谱均与T1的相同。结论经4种同工酶分析我国6个猪源旋毛虫地理株均为T1。

Objective To provide evidence for the classification of Trichinella. Methods Isoenzymes of superoside dismutase (SOD), malic enzyme (ME), esterase (EST) and glutamate dehydrogenase (GLDH) of muscle larvae of six swine Trichinella isolated from Henan, Yunnan, Hubei province, Tianjing and Harbin and Tongjiang of Heilongjiang were analyzed by PAGE. International reference strains of five Trichinella species [Trichinella spiralis (T1), T. nativa (T2), T. britovi (T3), T. pseudospiralis(T4)and T. nelsoni(T7)] were used as control strains. Results The electrophoretic patterns of the four isoenzymes from the six six swine Trichinella were the same as those of T1. Conclusion All of the six swine Trichinella isolates ate identified as T1 by isoenzymes analysis.